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Simona Mihai

Latest posts by Simona Mihai (see all)

  • DYNAMICS OF ENDOTHELIN LEVELS IN MUSCLE REGENERATION AFTER MECHANICAL TRAUMA - 19/11/2015
  • CD105/ENDOGLIN EXPRESSION IN A MOUSE MODEL OF ACUTE MUSCLE CONTUSION - 15/09/2015

Articles signed on Romanian Journal of NEUROLOGY:

DYNAMICS OF ENDOTHELIN LEVELS IN MUSCLE REGENERATION AFTER MECHANICAL TRAUMA

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Romanian Journal of Neurology, Volume XIV, No. 3, 2015
ISSN 1843-8148  |  e-ISSN 2069-6094
ISSN-L 1843-8148
DOI: 10.37897/RJN

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DYNAMICS OF ENDOTHELIN LEVELS IN MUSCLE REGENERATION AFTER MECHANICAL TRAUMA

Laura Cristina Ceafalan, Emilia Manole, Cristiana Pistol Tanase, Elena Codrici, Simona Mihai and Bogdan O. Popescu

ABSTRACT

Objectives. Muscle regeneration after trauma is a complex phenomenon involving several cellular processes, such as angiogenesis, inflammation, fibrosis, activation of satellite cells and their differentiation into myocytes and myotubes. Although many studies explored these mechanisms in the last years, there is still an unmet need to find new therapy targets, especially regarding some cellular molecules involved in muscular recovery after mechanical or pathological injury. In the present study we investigated the dynamics of endothelin-1 (ET-1), an important factor that has been shown to be involved in all stages of tissue regeneration, but which is poorly investigated in skeletal muscle.
Materials and methods. We used an experimental animal model of acute mechanical trauma on mouse gastrocnemius muscle. ET-1 levels were investigated at different time-points after muscle injury by in situ immunofluorescence, xMAP assay on tissue and serum samples, and Western Blot analysis.
Results. By xMAP assay, tissue ET-1 levels increased significantly up to the 5th day after trauma, correlated with serum levels. xMAP assay was confirmed by Western blot analysis which showed a significant increase in the level of ET-1 towards the end of the first week after trauma. This corresponds with the inflammatory stage of the regeneration process, followed by angiogenesis and satellite cell activation. In situ immunostaining showed a multiplication of interstitial cells expressing ET-1 in the first week after muscle injury. Two cellular subtypes were detected in the connective tissue – one is represented by blood-derived CD45 positive cells and the other by local interstitial cells. Such cells were detected in all connective tissue compartments, in close association with CD56 positive satellite cells, myoblasts and myotubes and most of them co-express sca-1.
Conclusions. The present study demonstrated that ET-1 is synthesized mostly by mesenchymal progenitors and their number greatly increases after mechanical trauma in muscle interstitium. Based on ET-1 expression and their close association with activated satellite cells, such cells could have a paracrine influence not only over angiogenesis but also over fiber regeneration. ET-1 appears as an important molecule working in conjunction with other various signalling pathways especially during first stages of the regeneration process after acute mechanical injury. ET-1 and its receptors could become therapeutic targets, especially for inflammatory myopathies and muscular dystrophies with significant pathological fibrosis.

Keywords: endothelin, skeletal muscle mechanical trauma, muscle regeneration, angiogenesis, inflammation, satellite cells, fibrosis

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CD105/ENDOGLIN EXPRESSION IN A MOUSE MODEL OF ACUTE MUSCLE CONTUSION

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Romanian Journal of Neurology, Volume XIII, No. 4, 2014
ISSN 1843-8148  |  e-ISSN 2069-6094
ISSN-L 1843-8148
DOI: 10.37897/RJN

Indexed

DOI - Crossref
Similarity Check by iThenticate, worldwide No 1 professional plagiarism checking system
DOAJ
Scopus
NLM Catalog
Ebsco Host - Medline
Google Academic
Semantic Scholar

HIGHLIGHTS

ICMJE- Recommendations

Read the Recommendations for the Conduct, Reporting, Editing, and Publication of Scholarly work in Medical Journals.

Promoting Global Health

The published medical research literature is a global public good. Medical journal editors have a social responsibility to promote global health by publishing, whenever possible, research that furthers health worldwide.

CD105/ENDOGLIN EXPRESSION IN A MOUSE MODEL OF ACUTE MUSCLE CONTUSION

Antoanela Curici, Elena Codrici, Simona Mihai, Cristiana Pistol Tanase, Bogdan Ovidiu Popescu and Laura Cristina Ceafalan

ABSTRACT

Objectives. Muscle contusion is the most common form of muscle injury and the muscle endogenous regenerative capacity can compensate for non-extensive damage. However, severe traumatic injuries may overcome this capacity. More effective therapeutic strategies are still needed, so the exploration of the molecular context during muscle regeneration might provide new insights.

Materials and methods. We investigated the expression pattern of a reputed angiogenic molecule, CD105/endoglin, by measuring the tissue and serum concentration by multiplex assay in normal and Dmdmdx dystrophic mice by following the distribution of the cellular sources by immunofluorescence in a mouse model of acute muscle contusion.

Results. Maximal tissue concentration in normal animals was obtained 48h post-injury, and then started to decline, with only one other significantly increased value 5 days after injury. In dystrophic mice, tissue levels were globally much higher than in normal animals and started rising 1h post-injury and were maintained elevated all along the regeneration process. In situ immunolabelling highlighted the increased positive population mostly in the inflammatory areas. Double staining separates distinct subsets based on hematopoietic marker CD45 and the endothelial marker CD31 co-expression in endoglin positive cells.

Conclusions. This study offers a timeline of endoglin expression during normal and pathologic muscle regeneration, providing evidence that the major wave corresponds to the inflammatory stage of muscle regeneration, deriving from multiple cellular sources such as endothelial cells, blood cells and also other interstitial cells that become activated during this process.

Keywords: angiogenesis, CD105, endoglin, muscle regeneration

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